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rnascope hi-plex assay  (Advanced Cell Diagnostics Inc)

 
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    Advanced Cell Diagnostics Inc rnascope hi-plex assay
    Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the <t>RNAscope</t> Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.
    Rnascope Hi Plex Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnascope hi-plex assay/product/Advanced Cell Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    rnascope hi-plex assay - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "dotdotdot : an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues"

    Article Title: dotdotdot : an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaa312

    Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the RNAscope Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.
    Figure Legend Snippet: Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the RNAscope Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.

    Techniques Used: Imaging, RNAscope, Multiplex Assay, Fluorescence, Hybridization, Sequencing, Amplification, Binding Assay, Confocal Microscopy



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    rnascope hi-plex assay  (Advanced Cell Diagnostics Inc)
    90
    Advanced Cell Diagnostics Inc rnascope hi-plex assay
    Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the <t>RNAscope</t> Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.
    Rnascope Hi Plex Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnascope hi-plex assay/product/Advanced Cell Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    rnascope hi-plex assay - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the RNAscope Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.

    Journal: Nucleic Acids Research

    Article Title: dotdotdot : an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues

    doi: 10.1093/nar/gkaa312

    Figure Lengend Snippet: Experimental workflows and imaging protocols for smFISH in mouse and human tissues. ( A ) Brains were extracted from wild-type (WT) mice 90 min following electroconvulsive seizures (ECS) or Sham treatment and sectioned on a cryostat. Gene targets were visualized with the RNAscope Multiplex Fluorescence V1 kit. RNAscope technology uses hybridization of two independent probes (double Z probes), referred to as a ‘ZZ pair,’ that must bind to the target sequence in tandem for signal amplification to proceed via the subsequent binding of preamplifiers, amplifiers, and fluorescent detection molecules. Approximately 5–30 ZZ pairs are designed for each target gene. After completion of the RNAscope V1 assay, slides are imaged in x , y and z -dimensions using confocal microscopy. ( B ) Fresh frozen post-mortem human tissue was sectioned on a cryostat and gene targets were visualized using the RNAscope Multiplex Fluorescence V2 kit. The V2 assay uses the same RNAscope technology with added TSA technology for customization of dyes/concentrations and the ability to include a fourth gene target. The V2 assay is also better suited for tissues with autofluorescence, such as post-mortem human brain tissue, which contains an abundance of highly autofluorescent lipofuscin granules. Multispectral imaging and linear unmixing were used to separate individual probe signals and lipofuscin autofluorescence. Lipofuscin signals served as a mask during downstream analysis to exclude pixels confounded by autofluorescence.

    Article Snippet: Finally, as chemistry for higher order multiplexing is rapidly coming online, we evaluated the performance of dotdotdot on higher order multiplexed images acquired using the RNAscope Hi-plex assay, which visualizes up to 12 gene targets in a single tissue slice with several rounds of hybridization and stripping ( ). dotdotdot faithfully segments fluorescent signals from 12 positive control probes against different 'house-keeping' genes in the same brain section of mouse tissue (image courtesy of Advanced Cell Diagnostics).

    Techniques: Imaging, RNAscope, Multiplex Assay, Fluorescence, Hybridization, Sequencing, Amplification, Binding Assay, Confocal Microscopy